Application notesTGR has extensive knowledge of signaling characteristics of major pathways in many cell lines, and here we aim to provide a resource for scientists to optimize cellular experiments. However, if your cell line or pathway of interest is not listed here contact us to ask one our scientists.
Featured productsAtaxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are serine/threonine kinases that regulate cell cycle checkpoints in response to DNA damage. ATR is activated by a variety of agents that can induce DNA damage, including UV light, and particular chemicals that inhibit DNA replication or directly modify DNA, whereas ATM is induced primarily by conditions that induce double strand breaks in the DNA, such as ionizing radiation. Upon induction, ATM/ATR regulate the activity of several diverse classes of protein, many of which have been directly implicated in cellular checkpoint signaling pathways. These include scaffolding proteins, regulatory proteins, and other kinases. Among the better understood ATM/ATR substrates are p53, Chk1 and Chk2. Located immediately downstream of ATM/ATR, parallel analysis of phosphorylation of a combination of Chk1, Chk2 and p53 provides an ideal platform for interrogating the induction of ATM/ATR-mediated signaling. See below for cell-based applications on ATM/ATR signaling.
Signaling targetsClick on the links below for cell-based applications for different signaling pathways and targets
JAK/STAT signaling pathway downloads Akt signaling pathway downloads Application Note: STAT signaling in THP-1 cells Poster: GPCR-mediated signaling Application Note: STAT signaling in TF-1 cells
MAPK signaling pathway downloads NF-kappaB signaling pathway downloads Poster: GPCR-mediated signaling Coming soon!
ATM/ATR signaling pathway downloads mTOR signaling pathway downloads Application Note: ATM/ATR signaling Coming soon! Datasheet: AlphaScreen Surefire p-Chk1 Datasheet: AlphaScreen SureFire p-Chk2 Datasheet: AlphaScreen SureFire p-p53 Datasheet: AlphaScreen SureFire total p53
TGFbeta signaling pathway downloads Other kits Datasheet: AlphaScreen SureFire p-Smad1 Datasheet: AlphaScreen SureFire p-Histone H3 Datasheet: AlphaScreen SureFire p-Smad2
Note: this list is regularly updated. Last update May 2010. |
