Scientific publicationsThe AlphaScreen® SureFire® technology has been used extensively to interrogate cellular signalling mechanisms. Below are some selected publications that highlight particular applications of the AlphaScreen SureFire assay technology. Click here for an extensive list of publications citing the use of AlphaScreen SureFire products.
ALPHASCREEN® SureFire® applications - Selected RECENT publicationsBinder C, Lafayette A, Archibeque I, Sun Y, Plewa C, Sinclair A, Emkey R (2008) Optimization and utilization of the surefire phospho-Stat5 assay for a cell-based screening campaign. Assay Drug Dev. Tech. 6, 27-37. Read more Gregory KJ, Hall NE, Tobin AB, Sexton PM, Christopoulos A (2010) Identification of orthosteric and allosteric site mutations in M2 muscarinic acetylcholine receptors that contribute to ligand-selective signaling bias. J. Biol. Chem. 285, 7459-7474. Read more Harrison RS, Shepherd NE, Hoang HN, Ruiz-Gómez G, Hill TA, Driver RW, Desai VS, Young PR, Abbenante G, Fairlie DP (2010) Downsizing human, bacterial, and viral proteins to short water-stable alpha helices that maintain biological potency. Proc. Natl. Acad. Sci. USA, 107, 11686-91. Read more Hay DL (2009) Measurement of phosphorylated Extracellular Signal–Regulated Kinase 1 and 2 in an undergraduate teaching laboratory with ALPHAscreen technology. Sci. Signal. 2, tr1. Read more Leach K, Loiacono RE, Felder CC, McKinzie DL, Mogg A, Shaw DB, Sexton PM, Christopoulos A (2010) Molecular mechanisms of action and In vivo validation of an M4 muscarinic acetylcholine receptor allosteric modulator with potential antipsychotic properties. Neuropharmacol. 35(4):855-69. Read more Lodge AP, Langmead CJ, Daniel G, Anderson G, Werry TD (2010) Performance of mouse neural stem cells as a screening reagent: Characterisation of PAC1 Activity in medium-throughput functional assays. J. Biol. Screen. 15, 159-168. Read more Smith NJ, Stoddart LA, Devine NM, Jenkins L, Milligan G (2009) The action and mode of binding of thiazolidinedione ligands at free fatty acid receptor 1. J. Biol. Chem. 284, 17527-17539. Read more Werden SJ, McFadden G (2010) Pharmacological manipulation of the Akt signaling pathway regulates myxoma virus replication and tropism in human cancer cells. J. Virol. 84, 3287-3302. Read more
Selected publications by TGR scientistsTGR staff also actively contribute to research interrogating kinase signalling mechanisms in areas such as GPCR biology and cancer research. Development and validation of cellular models for cellular signalling studies is a key area of interest. Osmond RIW, Crouch MF, Dupriez V (2010) An emerging role for kinase screening in GPCR drug discovery. Curr Opin Mol Ther. 12(3), 305-15. Read more Osmond RIW, Das S, Crouch MF. (2010) Development of cell based assays for cytokine receptor signaling, using an AlphaScreen SureFire assay format. Anal. Biochem. In Press. Read more Werry TD, Stewart GD, Crouch MF, Watts A, Sexton PM, Christopoulos A. (2008) Pharmacology of 5HT(2C) receptor-mediated ERK1/2 phosphorylation: agonist-specific activation pathways and the impact of RNA editing. Biochem. Pharmacol. 76, 1276-1287. Read more Crouch MF, Osmond RIW (2008) New strategies in drug discovery for GPCRs: high throughput detection of cellular ERK phosphorylation. Comb Chem High Throughput Screen. 11, 344-356. Read more Lee HJ, Mun HC, Lewis NC, Crouch MF, Culverston EL, Mason RS, Conigrave AD. (2007) Allosteric activation of the extracellular Ca2+-sensing receptor by L-amino acids enhances ERK1/2 phosphorylation. Biochem J. 404, 141-149. Read more Matthews CA, Shaw JE, Hooper JA, Young IG, Crouch MF, Campbell HD. (2007) Expression and evolution of the mammalian brain gene Ttyh1. J Neurochem. 100, 693-707. Read more Osmond RIW, Sheehan A, Borowicz R, Barnett E, Harvey G, Turner C, Brown A, Crouch MF, Dyer AR (2005) GPCR screening via ERK 1/2: a novel platform for screening G protein-coupled receptors. J. Biomol. Screen. 10, 730-737. Read more
Last updated: July 2010 |
